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Cloning and Expression
Enzymes
Alt-R S.p. dCas9 Protein V3
Alt-R S.p. dCas9 Protein V3 from Integrated DNA Technologies
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Citations:
(4)
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Integrated DNA Technologies for
Alt-R S.p. dCas9 Protein V3
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Description
Recombinant S. pyogenes dCas9 protein, purified from an E. coli strain expressing dCas9. Contains nuclear localization sequence (NLS) and C-terminal 6-His tag. Provided in solution at 10 µg/µL. 100 µg of dCas9 protein = 610 pmol
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Alt-R S.p. dCas9 Protein V3
Company
Integrated DNA Technologies
Price
Supplier Page
View Company Product Page
Catalog Number
1081066
Applications
genome editing, CRISPR
Type
Enzyme
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Integrated DNA Technologies
1710 Commercial Park
Coralville
,
Iowa
52241
United States
Phone
: 800-328-2661
Company Profile
Website:
http://www.idtdna.com
Citations (4)
(1)
Selection of Extended CRISPR RNAs with Enhanced Targeting and Specificity
bioRxiv
January 15, 2023
Ashley Herring-Nicholas, Hillary Dimig, Miranda Roesing, Eric A. Josephs
#CAS9PROT-250UG), eCas9 enzyme (Sigma Aldrich #ESPCAS9PRO-50UG), or dCas9 enzyme (
IDT
Alt-R® S.p. dCas9 Protein V3
#
1081066
) and sgRNA were combined in equimolar amounts in Phosphate buffered saline, pH 7.4 - PBS (
ThermoFisher
,
(2)
A genetically engineered, stem-cell-derived cellular vaccine
Cell reports. Medicine
December 20, 2022
Cooper A, Sidaway A, Chandrashekar A, Latta E, Chakraborty K, Yu J, McMahan K, Giffin V, Manickam C, Kroll K,
et al
.
and recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, Cas9 protein,
IDT
,Cat#
1081066
VEG-F protein,
Peprotech
,Cat#
100-20
Leukocyte
activation cocktail,
BD Biosciences
,Cat#
550583
Vitronectin,
StemCell Technologies
(3)
HP1 proteins compact DNA into mechanically and positionally stable phase separated domains
eLife
March 4, 2021
Madeline M Keenen, David Brown, Lucy D Brennan, Roman Renger, Harrison Khoo, Christopher R Carlson, Bo Huang, Stephan W Grill, Geeta J Narlikar, Sy Redding
during experiments. End-
labeling
of DNA was accomplished using dCas9 molecules. Specifically, dCas9 (
IDT
1081066
),
Alt-R CRISPR-Cas9 tracrRNA
(
IDT
1072532
), and an
Alt-R CRISPR-Cas9 crRNA
targeting
bacteriophage
λ
(4)
Single-strand DNA breaks cause replisome disassembly
Molecular Cell
March 1, 2021
Kyle B. Vrtis, James M. Dewar, Gheorghe Chistol, R. Alex Wu, Thomas G. W. Graham, Johannes C. Walter
S.p. Cas9 H840A Nickase V3, 100 μg",
IDT
,Cat#
1081064
"
Alt-R S.p. dCas9 Protein V3
, 100 μg",
IDT
,Cat#
1081066
Critical Commercial
Assays
,Critical Commercial
Assays
,Critical Commercial
Assays
Bac-to-Bac Baculovirus Expression System
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Alt-R S.p. dCas9 Protein V3 from Integrated DNA Technologies
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